Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture (2024)

‘Aizat Norhisham, D., Md Saad, N., Ahmad Usuldin, S. R., Vayabari, D. A. G., Ilham, Z., Ibrahim, M. F., Show, P. L., & Wan-Mohtar, W. A. A. Q. I. (2023). Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture. Bioengineered, 14(1), Article 2262203. https://doi.org/10.1080/21655979.2023.2262203

‘Aizat Norhisham, Danial ; Md Saad, Norsharina ; Ahmad Usuldin, Siti Rokhiyah et al. / Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture. In: Bioengineered. 2023 ; Vol. 14, No. 1.

@article{c5f3a4d9d5234d2b800ecf73c20c0161,

title = "Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture",

abstract = "The versatility of a well-known fibrous crop, Hibiscus cannabinus (kenaf) is still relatively new to many. Kenaf{\textquoteright}s potential applications, which can be extended even into critical industries such as pharmaceutical and food industries, have always been overshadowed by its traditionally grown fiber. Therefore, this study aimed to venture into the biotechnological approach in reaping the benefits of kenaf through plant cell suspension culture to maximize the production of kenaf callus biomass (KCB) and exopolysaccharide (EPS), which is deemed to be more sustainable. A growth curve was established which indicates that cultivating kenaf callus in suspension culture for 22 days gives the highest KCB (9.09 ± 1.2 g/L) and EPS (1.1 ± 0.02 g/L). Using response surface methodology (RSM), it was found that sucrose concentration, agitation speed, and naphthalene acetic acid (NAA) concentration can affect the production of KCB and EPS significantly (p < 0.05) while 2,4-dichlorophenoxy acetic acid (2,4-D) was deemed insignificant. To maximize the final yield of KCB and EPS, the final optimized variables are 50 g/L sucrose, 147.02 rpm, and 2 mg/L of NAA. To conclude, the optimized parameters for the cell suspension culture of kenaf callus serve as the blueprint for any sustainable large-scale production in the future and provide an alternative cultivating method to kenaf traditional farming.",

keywords = "bioreactor, exopolysaccharide, Hibiscus cannabinus, kenaf, Life on land, optimization",

author = "{{\textquoteleft}Aizat Norhisham}, Danial and {Md Saad}, Norsharina and {Ahmad Usuldin}, {Siti Rokhiyah} and Vayabari, {Diwiya A.G.} and Zul Ilham and Ibrahim, {Mohamad Faizal} and Show, {Pau Loke} and Wan-Mohtar, {Wan Abd Al Qadr Imad}",

note = "Publisher Copyright: {\textcopyright} 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.",

year = "2023",

doi = "10.1080/21655979.2023.2262203",

language = "British English",

volume = "14",

journal = "Bioengineered",

issn = "2165-5979",

publisher = "Taylor and Francis Ltd.",

number = "1",

}

‘Aizat Norhisham, D, Md Saad, N, Ahmad Usuldin, SR, Vayabari, DAG, Ilham, Z, Ibrahim, MF, Show, PL & Wan-Mohtar, WAAQI 2023, 'Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture', Bioengineered, vol. 14, no. 1, 2262203. https://doi.org/10.1080/21655979.2023.2262203

Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture. / ‘Aizat Norhisham, Danial; Md Saad, Norsharina; Ahmad Usuldin, Siti Rokhiyah et al.
In: Bioengineered, Vol. 14, No. 1, 2262203, 2023.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture

AU - ‘Aizat Norhisham, Danial

AU - Md Saad, Norsharina

AU - Ahmad Usuldin, Siti Rokhiyah

AU - Vayabari, Diwiya A.G.

AU - Ilham, Zul

AU - Ibrahim, Mohamad Faizal

AU - Show, Pau Loke

AU - Wan-Mohtar, Wan Abd Al Qadr Imad

N1 - Publisher Copyright:© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

PY - 2023

Y1 - 2023

N2 - The versatility of a well-known fibrous crop, Hibiscus cannabinus (kenaf) is still relatively new to many. Kenaf’s potential applications, which can be extended even into critical industries such as pharmaceutical and food industries, have always been overshadowed by its traditionally grown fiber. Therefore, this study aimed to venture into the biotechnological approach in reaping the benefits of kenaf through plant cell suspension culture to maximize the production of kenaf callus biomass (KCB) and exopolysaccharide (EPS), which is deemed to be more sustainable. A growth curve was established which indicates that cultivating kenaf callus in suspension culture for 22 days gives the highest KCB (9.09 ± 1.2 g/L) and EPS (1.1 ± 0.02 g/L). Using response surface methodology (RSM), it was found that sucrose concentration, agitation speed, and naphthalene acetic acid (NAA) concentration can affect the production of KCB and EPS significantly (p < 0.05) while 2,4-dichlorophenoxy acetic acid (2,4-D) was deemed insignificant. To maximize the final yield of KCB and EPS, the final optimized variables are 50 g/L sucrose, 147.02 rpm, and 2 mg/L of NAA. To conclude, the optimized parameters for the cell suspension culture of kenaf callus serve as the blueprint for any sustainable large-scale production in the future and provide an alternative cultivating method to kenaf traditional farming.

AB - The versatility of a well-known fibrous crop, Hibiscus cannabinus (kenaf) is still relatively new to many. Kenaf’s potential applications, which can be extended even into critical industries such as pharmaceutical and food industries, have always been overshadowed by its traditionally grown fiber. Therefore, this study aimed to venture into the biotechnological approach in reaping the benefits of kenaf through plant cell suspension culture to maximize the production of kenaf callus biomass (KCB) and exopolysaccharide (EPS), which is deemed to be more sustainable. A growth curve was established which indicates that cultivating kenaf callus in suspension culture for 22 days gives the highest KCB (9.09 ± 1.2 g/L) and EPS (1.1 ± 0.02 g/L). Using response surface methodology (RSM), it was found that sucrose concentration, agitation speed, and naphthalene acetic acid (NAA) concentration can affect the production of KCB and EPS significantly (p < 0.05) while 2,4-dichlorophenoxy acetic acid (2,4-D) was deemed insignificant. To maximize the final yield of KCB and EPS, the final optimized variables are 50 g/L sucrose, 147.02 rpm, and 2 mg/L of NAA. To conclude, the optimized parameters for the cell suspension culture of kenaf callus serve as the blueprint for any sustainable large-scale production in the future and provide an alternative cultivating method to kenaf traditional farming.

KW - bioreactor

KW - exopolysaccharide

KW - Hibiscus cannabinus

KW - kenaf

KW - Life on land

KW - optimization

UR - http://www.scopus.com/inward/record.url?scp=85173019066&partnerID=8YFLogxK

U2 - 10.1080/21655979.2023.2262203

DO - 10.1080/21655979.2023.2262203

M3 - Article

C2 - 37791464

AN - SCOPUS:85173019066

SN - 2165-5979

VL - 14

JO - Bioengineered

JF - Bioengineered

IS - 1

M1 - 2262203

ER -

‘Aizat Norhisham D, Md Saad N, Ahmad Usuldin SR, Vayabari DAG, Ilham Z, Ibrahim MF et al. Performance of Malaysian kenaf Hibiscus cannabinus callus biomass and exopolysaccharide production in a novel liquid culture. Bioengineered. 2023;14(1):2262203. doi: 10.1080/21655979.2023.2262203