Mouse Gene Siglece (ENSMUST00000032667.9) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
| Description: Mus musculus sialic acid binding Ig-like lectin E (Siglece), mRNA. (from RefSeq NM_031181) Gencode Transcript: ENSMUST00000032667.9 Gencode Gene: ENSMUSG00000030474.9 Transcript (Including UTRs) Position:mm10 chr7:43,651,070-43,660,161Size:9,092Total Exon Count:7Strand:- Coding Region Position:mm10 chr7:43,651,486-43,660,132Size:8,647Coding Exon Count:7
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Sequence and Links to Tools and Databases
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Comments and Description Text from UniProtKB
| ID: SIG12_MOUSE DESCRIPTION: RecName: Full=Sialic acid-binding Ig-like lectin 12; Short=Siglec-12; AltName: Full=Myeloid inhibitory siglec; Short=MIS; AltName: Full=Sialic acid-binding Ig-like lectin 5; Short=Siglec-5; AltName: Full=Sialic acid-binding Ig-like lectin E; Short=Siglec-E; Short=mSiglec-E; AltName: Full=Sialic acid-binding Ig-like lectin-like 1; Short=Siglec-L1; Flags: Precursor; FUNCTION: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. SUBUNIT: hom*odimer; disulfide-linked. Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed by monocytic/myeloid lineage cells. Found at higher levels in spleen, liver and heart. Found at lower levels in kidney and lung. DOMAIN: Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2- containing phosphatases. PTM: Phosphorylation of Tyr-432 is required for binding to PTPN6 and PTPN11. Phosphorylation of Tyr-455 is involved in binding to PTPN6. Tyr-432 needs to be phosphorylated prior to Tyr-455. SIMILARITY: Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SIMILARITY: Contains 1 Ig-like V-type (immunoglobulin-like) domain. WEB RESOURCE: Name=Functional Glycomics Gateway - Glycan Binding; Note=Siglec-E; URL="http://www.functionalglycomics.org/glycomics/GBPServlet?&operationType=view&cbpId=cbp_mou_Itlect_197"; |
Primer design for this transcript
Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA. Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence. To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3 |
mRNA Secondary Structure of 3' and 5' UTRs
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have. | |||||||||||||||||||||||
Protein Domain and Structure Information
| InterPro Domains: Graphical view of domain structure IPR007110 - Ig-like IPR013783 - Ig-like_fold IPR003006 - Ig/MHC_CS IPR003599 - Ig_sub IPR003598 - Ig_sub2 IPR013106 - Ig_V-set Pfam Domains: SCOP Domains: ModBase Predicted Comparative 3D Structure on Q91Y57
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
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Gene Ontology (GO) Annotations with Structured Vocabulary
| Molecular Function: GO:0005057 signal transducer activity, downstream of receptor GO:0005515 protein binding GO:0030246 carbohydrate binding GO:0033691 sialic acid binding GO:0038023 signaling receptor activity GO:0048029 monosaccharide binding Biological Process: Cellular Component: |
Descriptions from all associated GenBank mRNAs
| AF317298 - Mus musculus sialic acid-binding Ig-like lectin mRNA, complete cds. BC023280 - Mus musculus sialic acid binding Ig-like lectin E, mRNA (cDNA clone MGC:30592 IMAGE:3662856), complete cds. AF329269 - Mus musculus myeloid inhibitory siglec mRNA, complete cds. AY371487 - Mus musculus sialic acid binding Ig-like lectin E mRNA, complete cds. AY348552 - Mus musculus sialic acid binding immunoglobulin-like lectin-E mRNA, complete cds. |
Biochemical and Signaling Pathways
| Reactome (by CSHL, EBI, and GO) Protein Q91Y57 (Reactome details) participates in the following event(s): R-MMU-5685607 Sialic acid binds SIGLEC |
| Alternate Gene Symbols: E9QMD1, NM_031181, Q91Y57, SIG12_MOUSE, Siglec12, Siglec5, Siglecl1, uc009gng.1 UCSC ID: uc009gng.1 RefSeq Accession: NM_031181 Protein: Q91Y57 (aka SIG12_MOUSE) CCDS: CCDS21178.1 |
