520-0.288 525-0.273 530-0.251 535-0.288 540-0.2 545-0.167 550-0.147 555-0.118 560-0.097 Part II: Calibration Curve data Concentration (M) Absorbance value 0.25M-1.15 0.20M-0.9 0.125M-0.51 0.0625M-0.197 0.005M-0.13 Absorbance of Unknown solution = 0.486 (2024)

`); let searchUrl = `/search/`; history.forEach((elem) => { prevsearch.find('#prevsearch-options').append(`

${elem}

`); }); } $('#search-pretype-options').empty(); $('#search-pretype-options').append(prevsearch); let prevbooks = $(false); [ {title:"Recently Opened Textbooks", books:previous_books}, {title:"Recommended Textbooks", books:recommended_books} ].forEach((book_segment) => { if (Array.isArray(book_segment.books) && book_segment.books.length>0 && nsegments<2) { nsegments+=1; prevbooks = $(`

  • ${book_segment.title}
  • `); let searchUrl = "/books/xxx/"; book_segment.books.forEach((elem) => { prevbooks.find('#prevbooks-options'+nsegments.toString()).append(`

    ${elem.title} ${ordinal(elem.edition)} ${elem.author}

    `); }); } $('#search-pretype-options').append(prevbooks); }); } function anon_pretype() { let prebooks = null; try { prebooks = JSON.parse(localStorage.getItem('PRETYPE_BOOKS_ANON')); }catch(e) {} if ('previous_books' in prebooks && 'recommended_books' in prebooks) { previous_books = prebooks.previous_books; recommended_books = prebooks.recommended_books; if (typeof PREVBOOKS !== 'undefined' && Array.isArray(PREVBOOKS)) { new_prevbooks = PREVBOOKS; previous_books.forEach(elem => { for (let i = 0; i < new_prevbooks.length; i++) { if (elem.id == new_prevbooks[i].id) { return; } } new_prevbooks.push(elem); }); new_prevbooks = new_prevbooks.slice(0,3); previous_books = new_prevbooks; } if (typeof RECBOOKS !== 'undefined' && Array.isArray(RECBOOKS)) { new_recbooks = RECBOOKS; for (let j = 0; j < new_recbooks.length; j++) { new_recbooks[j].viewed_at = new Date(); } let insert = true; for (let i=0; i < recommended_books.length; i++){ for (let j = 0; j < new_recbooks.length; j++) { if (recommended_books[i].id == new_recbooks[j].id) { insert = false; } } if (insert){ new_recbooks.push(recommended_books[i]); } } new_recbooks.sort((a,b)=>{ adate = new Date(2000, 0, 1); bdate = new Date(2000, 0, 1); if ('viewed_at' in a) {adate = new Date(a.viewed_at);} if ('viewed_at' in b) {bdate = new Date(b.viewed_at);} // 100000000: instead of just erasing the suggestions from previous week, // we just move them to the back of the queue acurweek = ((new Date()).getDate()-adate.getDate()>7)?0:100000000; bcurweek = ((new Date()).getDate()-bdate.getDate()>7)?0:100000000; aviews = 0; bviews = 0; if ('views' in a) {aviews = acurweek+a.views;} if ('views' in b) {bviews = bcurweek+b.views;} return bviews - aviews; }); new_recbooks = new_recbooks.slice(0,3); recommended_books = new_recbooks; } localStorage.setItem('PRETYPE_BOOKS_ANON', JSON.stringify({ previous_books: previous_books, recommended_books: recommended_books })); build_popup(); } } var whiletyping_search_object = null; var whiletyping_search = { books: [], curriculum: [], topics: [] } var single_whiletyping_ajax_promise = null; var whiletyping_database_initial_burst = 0; //number of consecutive calls, after 3 we start the 1 per 5 min calls function get_whiletyping_database() { //gets the database from the server. // 1. by validating against a local database value we confirm that the framework is working and // reduce the ammount of continuous calls produced by errors to 1 per 5 minutes. return localforage.getItem('whiletyping_last_attempt').then(function(value) { if ( value==null || (new Date()) - (new Date(value)) > 1000*60*5 || (whiletyping_database_initial_burst < 3) ) { localforage.setItem('whiletyping_last_attempt', (new Date()).getTime()); // 2. Make an ajax call to the server and get the search database. let databaseUrl = `/search/whiletype_database/`; let resp = single_whiletyping_ajax_promise; if (resp === null) { whiletyping_database_initial_burst = whiletyping_database_initial_burst + 1; single_whiletyping_ajax_promise = resp = new Promise((resolve, reject) => { $.ajax({ url: databaseUrl, type: 'POST', data:{csrfmiddlewaretoken: "6SevNXy1yXJ5Huecb1MFaX7VZktRCnEsxa0oOOWFYRhIGwXBQwzC700uKfPyXLgC"}, success: function (data) { // 3. verify that the elements of the database exist and are arrays if ( ('books' in data) && ('curriculum' in data) && ('topics' in data) && Array.isArray(data.books) && Array.isArray(data.curriculum) && Array.isArray(data.topics)) { localforage.setItem('whiletyping_last_success', (new Date()).getTime()); localforage.setItem('whiletyping_database', data); resolve(data); } }, error: function (error) { console.log(error); resolve(null); }, complete: function (data) { single_whiletyping_ajax_promise = null; } }) }); } return resp; } return Promise.resolve(null); }).catch(function(err) { console.log(err); return Promise.resolve(null); }); } function get_whiletyping_search_object() { // gets the fuse objects that will be in charge of the search if (whiletyping_search_object){ return Promise.resolve(whiletyping_search_object); } database_promise = localforage.getItem('whiletyping_database').then(function(database) { return localforage.getItem('whiletyping_last_success').then(function(last_success) { if (database==null || (new Date()) - (new Date(last_success)) > 1000*60*60*24*30 || (new Date('2023-04-25T00:00:00')) - (new Date(last_success)) > 0) { // New database update return get_whiletyping_database().then(function(new_database) { if (new_database) { database = new_database; } return database; }); } else { return Promise.resolve(database); } }); }); return database_promise.then(function(database) { if (database) { const options = { isCaseSensitive: false, includeScore: true, shouldSort: true, // includeMatches: false, // findAllMatches: false, // minMatchCharLength: 1, // location: 0, threshold: 0.2, // distance: 100, // useExtendedSearch: false, ignoreLocation: true, // ignoreFieldNorm: false, // fieldNormWeight: 1, keys: [ "title" ] }; let curriculum_index={}; let topics_index={}; database.curriculum.forEach(c => curriculum_index[c.id]=c); database.topics.forEach(t => topics_index[t.id]=t); for (j=0; j

    Solutions
  • Textbooks
  • `); } function build_solutions() { if (Array.isArray(solution_search_result)) { const viewAllHTML = userSubscribed ? `View All` : ''; var solutions_section = $(`
  • Solutions ${viewAllHTML}
  • `); let questionUrl = "/questions/xxx/"; let askUrl = "/ask/question/xxx/"; solution_search_result.forEach((elem) => { let url = ('course' in elem)?askUrl:questionUrl; let solution_type = ('course' in elem)?'ask':'question'; let subtitle = ('course' in elem)?(elem.course??""):(elem.book ?? "")+"    "+(elem.chapter?"Chapter "+elem.chapter:""); solutions_section.find('#whiletyping-solutions').append(` ${elem.text} ${subtitle} `); }); $('#search-solution-options').empty(); if (Array.isArray(solution_search_result) && solution_search_result.length>0){ $('#search-solution-options').append(solutions_section); } MathJax.typesetPromise([document.getElementById('search-solution-options')]); } } function build_textbooks() { $('#search-pretype-options').empty(); $('#search-pretype-options').append($('#search-solution-options').html()); if (Array.isArray(textbook_search_result)) { var books_section = $(`
  • Textbooks View All
  • `); let searchUrl = "/books/xxx/"; textbook_search_result.forEach((elem) => { books_section.find('#whiletyping-books').append(` ${elem.title} ${ordinal(elem.edition)} ${elem.author} `); }); } if (Array.isArray(textbook_search_result) && textbook_search_result.length>0){ $('#search-pretype-options').append(books_section); } } function build_popup(first_time = false) { if ($('#search-text').val()=='') { build_pretype(); } else { solution_and_textbook_search(); } } var search_text_out = true; var search_popup_out = true; const is_login = false; const user_hash = null; function pretype_setup() { $('#search-text').focusin(function() { $('#search-popup').addClass('show'); resize_popup(); search_text_out = false; }); $( window ).resize(function() { resize_popup(); }); $('#search-text').focusout(() => { search_text_out = true; if (search_text_out && search_popup_out) { $('#search-popup').removeClass('show'); } }); $('#search-popup').mouseenter(() => { search_popup_out = false; }); $('#search-popup').mouseleave(() => { search_popup_out = true; if (search_text_out && search_popup_out) { $('#search-popup').removeClass('show'); } }); $('#search-text').on("keyup", delay(() => { build_popup(); }, 200)); build_popup(true); let prevbookUrl = `/search/pretype_books/`; let prebooks = null; try { prebooks = JSON.parse(localStorage.getItem('PRETYPE_BOOKS_'+(is_login?user_hash:'ANON'))); }catch(e) {} if (prebooks && 'previous_books' in prebooks && 'recommended_books' in prebooks) { if (is_login) { previous_books = prebooks.previous_books; recommended_books = prebooks.recommended_books; if (prebooks.time && new Date().getTime()-prebooks.time<1000*60*60*6) { build_popup(); return; } } else { anon_pretype(); return; } } $.ajax({ url: prevbookUrl, method: 'POST', data:{csrfmiddlewaretoken: "6SevNXy1yXJ5Huecb1MFaX7VZktRCnEsxa0oOOWFYRhIGwXBQwzC700uKfPyXLgC"}, success: function(response){ previous_books = response.previous_books; recommended_books = response.recommended_books; if (is_login) { localStorage.setItem('PRETYPE_BOOKS_'+user_hash, JSON.stringify({ previous_books: previous_books, recommended_books: recommended_books, time: new Date().getTime() })); } build_popup(); }, error: function(response){ console.log(response); } }); } $( document ).ready(pretype_setup); $( document ).ready(function(){ $('#search-popup').on('click', '.search-view-item', function(e) { e.preventDefault(); let autoCompleteSearchViewUrl = `/search/autocomplete_search_view/`; let objectUrl = $(this).attr('href'); let selectedId = $(this).data('objid'); let searchResults = []; $("#whiletyping-solutions").find("a").each(function() { let is_selected = selectedId === $(this).data('objid'); searchResults.push({ objectId: $(this).data('objid'), contentType: $(this).data('contenttype'), category: $(this).data('category'), selected: is_selected }); }); $("#whiletyping-books").find("a").each(function() { let is_selected = selectedId === $(this).data('objid'); searchResults.push({ objectId: $(this).data('objid'), contentType: $(this).data('contenttype'), category: $(this).data('category'), selected: is_selected }); }); $.ajax({ url: autoCompleteSearchViewUrl, method: 'POST', data:{ csrfmiddlewaretoken: "6SevNXy1yXJ5Huecb1MFaX7VZktRCnEsxa0oOOWFYRhIGwXBQwzC700uKfPyXLgC", query: $('#search-text').val(), searchObjects: JSON.stringify(searchResults) }, dataType: 'json', complete: function(data){ window.location.href = objectUrl; } }); }); });
    520-0.288
525-0.273
530-0.251
535-0.288
540-0.2
545-0.167
550-0.147
555-0.118
560-0.097
Part II: Calibration Curve data
Concentration (M) Absorbance value 
0.25M-1.15
0.20M-0.9
0.125M-0.51
0.0625M-0.197
0.005M-0.13
Absorbance of Unknown solution = 0.486 (2024)

    FAQs

    How to do a beer's law plot? ›

    To construct a Beer's law plot we prepare a series of standard solutions—each of which contains a known total concentration of HA—and then measure each solution's absorbance at the same wavelength.

    What should you do if you measure the absorbance of a solution and it is outside of the beer's law range? ›

    If the absorbance of your unknown is out of range, use volumetric glassware and graduated pipets to prepare a diluted unknown solution that has an absorbance in range of your calibration plot. (You may have to try this more than once.

    How to use a calibration equation? ›

    The equation will be of the general form y = mx + b, where m is the slope and b is the y-intercept, such as y = 1.05x + 0.2. Use the equation of the calibration curve to adjust measurements taken on samples with unknown values. Substitute the measured value as x into the equation and solve for y (the “true” value).

    What does a good calibration curve look like? ›

    The calibration curve is a plot of instrumental signal vs. concentration. The plot of the standards should be linear, and can be fit with the equation y=mx+b. The non-linear portions of the plot should be discarded, as these concentration ranges are out of the limit of linearity.

    Why calibration curves in HPLC? ›

    It is an experimentally generated graph with the concentration of solution plotted on the x-axis and the observable on the y-axis. Calibration curves are primarily used to determine the concentration of an unknown sample.

    Should a calibration curve go through 0? ›

    For compounds that do have some background, forcing through zero can help to show the concentration of your background. Often though, forcing the calibration through zero can give very poor results for the lower end of the calibration curve, which becomes more pronounced as the background increases.

    What is the Lambert Beer law? ›

    The Beer-Lambert law states that there is a linear relationship between the concentration and the absorbance of the solution, which enables the concentration of a solution to be calculated by measuring its absorbance.

    What is lambda max? ›

    Lambda max (λmax): The wavelength at which a substance has its strongest photon absorption (highest point along the spectrum's y-axis).

    What is a good R2 value for a calibration curve? ›

    The r or r2 values that accompany our calibration curve are measurements of how closely our curve matches the data we have generated. The closer the values are to 1.00, the more accurately our curve represents our detector response. Generally, r values ≥0.995 and r2 values ≥ 0.990 are considered 'good'.

    What is the 4 to 1 rule in calibration? ›

    A 4:1 TUR is the point to which most high-quality calibration labs strive. It is the point at which the level of in-tolerance probability stays at 100% the longest, with the best economies of scale.

    What is a good slope for a calibration curve? ›

    good calibration (calibration slope 0.95).” “The model was ... well calibrated (calibration slope, 1).”

    How do you interpret calibration slope? ›

    The calibration slope evaluates the spread of the estimated risks and has a target value of 1. A slope < 1 suggests that estimated risks are too extreme, i.e., too high for patients who are at high risk and too low for patients who are at low risk.

    How do you define calibration curve? ›

    The calibration curve is a plot of how the instrumental response, the so-called analytical signal, changes with the concentration of the analyte (the substance to be measured). A calibration curve plot showing limit of detection (LOD), limit of quantification (LOQ), dynamic range, and limit of linearity (LOL).

    What is a good calibration curve r2? ›

    The r or r2 values that accompany our calibration curve are measurements of how closely our curve matches the data we have generated. The closer the values are to 1.00, the more accurately our curve represents our detector response. Generally, r values ≥0.995 and r2 values ≥ 0.990 are considered 'good'.

    How do you use a calibration curve to determine concentration? ›

    How to calculate concentration from the calibration curve? Here you subtract the background b (the effect of the matrix) from the signal y, and then you divide by the sensitivity of the instrument used, a. The result is the concentration, x, with units depending on the technique with which the analysis is performed.

    References

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